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Image Search Results
Journal: BMC Genomics
Article Title: AlgaGEM – a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome
doi: 10.1186/1471-2164-12-S4-S5
Figure Lengend Snippet: The process for genome-scale model reconstruction. (1) Metabolic properties including associations between genes, enzymes and reactions were extracted from the genomic metabolic databases, stored and curated in an Excel spread-sheet; (2) a 2D reaction centric SBML representation was generated using an in house java application. (3) The stoichiometric matrix, as well as reversibility constraints and the objective function were extracted from the SBML; (4) the relevant linear programming problems were solved using the COBRA toolbox . (5) The model was refined in an iterative process, accessing the best available information in the literature and online data sources to achieve metabolic functionality.
Article Snippet: Constraint-based reconstruction and analysis was performed using
Techniques: Generated, Combined Bisulfite Restriction Analysis Assay